Tube ID	0.1 ug/uL l DNA	10x buffer	10 U/uL enzyme	dH2O
B	5 uL	2 uL E	1 uL BamHI	12 uL
E	5 uL	2 uL H	1 uL EcoRI	12 uL
H	5 uL	2 uL E	1 uL HindIII	12 uL
BH	5 uL	2 uL E	1 uL BamHI+1 uL HindIII	11 uL
EH	5 uL	2 uL MC*	1 uL EcoRI+1 uL HindIII	11 uL
BE	5 uL	2 uL MC*	1 uL BamHI+1 uL EcoRI	11 uL

• MC = Promega's "multicore" buffer with the white cap

• Set up the digests. We need two sets of digests, or 12 tubes total.
• Add correct amount of dH₂O
• Add 5 uL of Lambda DNA to each tube
• Use a fresh tip to add the correct 10x buffer
• Use a fresh tip to add the correct enzyme or enzymes.
• Put tubes into the 37'C waterbath and note the start time for the digests.
• Tubes should digest for one hour