PROTOCOL SUMMARY - PROBE DETECTION
| STEP | BUFFER | AGITATION | TIME | TEMP |
| 1 | 80~100 mL wash | shaking | 5 min | room temp |
| 2 | 50~60 mL wash | shaking | 5 min | room temp |
| 3 | 50~60 mL wash | shaking | 5 min | 50'C |
| 4 | 80~100 mL buffer #1 | shaking | 5 min | room temp |
| 5 | 80~100 mL buffer #2 | shaking | 15 min | room temp |
| 6 | 40 mL Ab/enz sol'n | shaking | 15 min | room temp |
| 7 | 80~100 mL buffer #1 | shaking | 5 min | room temp |
| 8 | 50~60 mL buffer #1 | shaking | 5 min | room temp |
| 9 | 80~100 mL buffer #3 | shaking | 5 min | room temp |
pour off buffer #3, transfer to another container, add 20 mL freshly-prepared developing sol'n in the dark