Gel #1: identify the sc, linear, and nc forms of uncut pAMP DNA. Can you identify the four darkest bands near the top of the NAC M1(+) digest?
Gel #2: compare SPG M1(-) with KG M1(-). Why is there a size difference? Why do the SPG M1(+) and KG M1(+) lanes look the same? Identify as many bands as possible in the MM M1(+) lane.
Gel #3: is ABM 27C(+) a positive clone? Why?
Gel #4: draw the plasmid represented by the bands in the MP M2(+) lane. Examine MM M2(+) and MP M1(+). Both of these lanes have only three bands. Can you suggest a possible plasmid represented by the bands in these two digests?