The Molecular Biology Lab Home Page

Department of Biology, College of Charleston

Experimental Molecular Biology


restriction digests of Lambda DNA	the double helix	minipreparation of plasmid pAMP DNA

announcements

the miniprep gel pictures will be posted before class - go to "Class Gel Photos" below and click on your lab day. Mon and Tue lab pictures are availalbe now; Wed lab gel pictures are coming soon - stay tuned.

NOTE schedule changes for lab periods 5 and 7; see outline below.

Instructor: Dr. Eileen McGuffie (E-mail: mcguffiee@cofc.edu).

Molecular Biology Lab Fall 2005 Syllabus

Check the Virtual Lab Book for Web readings and images

Handouts for lab period:

Lab period 1

Safety Handout
Expt. 1, 2, 7a Outline
Virtual Lab Book Reading on bacteriology for the molecular biologist
Virtual Lab Book images: plasmid antibiotic resistance , how to streak a plate , and streak plate examples

Lab period 2 (Agarose gel electrophoresis)

Agarose Gel Guide
Expt. 7b Outline
Virtual Lab Book Reading on restriction enzymes
Virtual Lab Book images on loading an agarose gel

Lab period 3 (Competent cells and transformation)

Expt. 8a and 8b Outline
Virtual Lab Book Reading: Using controls in Molecular Biology
Virtual Lab Book images on how to spread a plate and spreading examples

Lab period 4 (Minipreps and restriction analysis)

Expt. 10a and 10b Outline
Virtual Lab Book Understanding plasmid DNA
other readings to be announced

Lab period 5

Outline of Lambda digests for Southern analysis
Questions about previous pAK minipreps

Lab period 6 (MIDTERM Exam)

Lab period 7 (PCR of unknown bacteria)

Virtual Lab Book Reading on polymerase chain reaction
Virtual lab Book Images of PCR files

Lab period 8 (Fall Break - no lab)

Lab period 9 (Detection of ALU)

Outline for ALU detection using PCR

Lab period 10 (Southern analysis, partI)

Images on Southern analysis

Lab period 11 (Southern analysis, part II)

Summary of Probe Detection

Lab period 12 (Poster session)

Lab period 13 (Thanksgiving - no lab)

Lab Period 14 (FINAL exam)

Information on Molecular Biology Lab Scientific Posters:

Poster Guidelines

Study guide for:

Midterm Exam
Final Exam

CLASS GEL PHOTOS

Human DNA typing by the Molecular Biology Lab:
Determination of an ALU insertion polymorphism by PCR

MON LAB

TUE LAB

WED LAB

Spring 2004 Molecular Biology Lab

Poster Session

Using the yeast two-hybrid system to test mutations introduced into a viral transcription factor:
Sample results

Poster Pictures

Wednesday Lab

Thursday Lab

Friday Lab

Project Summary

A few useful Molecular Biology links:

Molecular Biology Lecture

Promega® catalog

New England Biolabs® (NEB) catalog


Department of Biology	College of Charleston