The Virtual Lab Book by Dr. Stephanie Dellis College of Charleston

Introduction

Some sections of the Lab Book are image-intensive, and may be frustrating to use with slower modems. I have tried to keep the images at each link to a minimum. However one result of this is more links. The Virtual Lab Book images are meant as a visual on-line resource and are not really designed for printing out. The "readings" however are meant to be printed.
 

Contents

• Reading:  Bacteriology for the Molecular Biologist

• plasmid antibiotic resistance

•  how to streak a plate

•  streak plate examples:  "the good, the bad, and the ugly"

•  how to spread a plate

•  spreading examples

II.  restriction digestion and agarose gel electrophoresis:

• Reading:  Restriction Enzymes

•  loading an agarose gel

III. plasmid DNA:

• Reading:  Understanding Plasmid DNA

IV. proper controls for Molecular Biology:

• Reading:  Controls in Molecular Biology

V.  polyacrylamide gel electrophoresis:

•  preparing the acrylamide gel

 
•  assembling the gel apparatus

VI.  polymerase chain reaction:

•  Reading: PCR and Thermalcycling

•  thermalcycler files

•  preparing the agarose gel

•  setting up the buffer tank and wick

•   applying the gel and preparing the membrane

•  setting up the transfer apparatus

•  final results