Assembling the buffer tank and wick

A wicking system is assembled that will draw 10x SSC buffer from a well in a large pan up to the gel. The gel will sit on the wicks on top of an "island" made by inverting a smaller pan.
 

First, pour buffer into the large pan. Then, carefully wet the wicks. Once the 3 mm filter paper is wet it can tear easily. 

Next, invert the small pan and place into the larger pan. Finally, carefully center the wicks over the small pan. Make sure there is enough wick on each side to reach into the buffer well.
Carefully tuck the wick into the buffer wells.

At this point, the gel has been denatured and neutralized and all the materials have been cut to size. 

The next step is placing the gel on the filter paper wick.

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