| First step: gently resuspend the cells and remove an aliquot. Remember do not grab these tubes by the caps. |  |
| Step two: squirt the bacteria into the center of
the petri plate.
Streak one plate at a time; do not allow the culture to soak into the center of the plate. |
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| Step three: sterilize the spreader in the alcohol.
Drip off the extra alcohol before flaming the spreader.
Keep the spreader pointed down during flaming! You do not want burning alcohol running onto your hand. |
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| Step four: once the flame is out, touch the spreader to the agar to be sure it is cool. Then spread in a circular motion until ALL the liquid is absorbed! This is the critical point to obtain isolated colonies. Spread around the outside of the plate as well as the center. |  |